We obtained a total of 25 (M. splendens) and 33 (O. curtisi) polymorphic bands (Tables 1 and 2). Every sample produced a different haplotype. The average gene diversity values ranged from 0.38 in M. splendens to 0.47 in O. curtisii. (Shannon's index=0.57 and 0.66, respectively). At a population level, both gene diversity and Shannon's index were lowest for M. splendens from Lerez/Albarellos and highest for O. curtisii from Lerez (Table 1). Sample size was positively correlated with both Nei's genetic diversity and Shannon's index (Pearson's r=0.81, and 0.80, respectively). The degree of population differentiation, as revealed by significant Fst values, varied from moderate (0.12, O. curtisii Lerez-Deza) to high (0.48, M. splendens Lerez-Cabe) (Tables 3 and 4). Within-population variation accounted for most of the total variation in both species (P < 0.001, Table 5), regardless of grouping criteria such as ecosystem or phylogeny.
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