Sampling and DNA Extraction

A total of 68 samples were analyzed in this study. Forty eight of these samples were obtained from collections in the Universities of York, United Kingdom, and Lausanne, Switzerland. Live pygmy shrews were also sampled in France (n = 5), including the island of Belle Île (n =10), using Trip Traps (Procter Bros. Ltd.). Details of sample locations are given in Table 1. DNA was extracted from ethanol-preserved tissue from tail tips, toe clippings or liver using the DNeasy Blood & Tissue Kit (Qiagen Ltd.) according to the manufacturer's protocol.

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