Buffers and Diluents

1. Wash buffer: 1X PBS solution containing protein and detergent used for wash steps and to resuspend beads for analysis. Store at 4°C.

2. Assay diluent: buffered solution used to dilute the cell signaling standards and to dilute test samples. Store at 4°C.

3. 5X Denaturation buffer: 5X sodium dodecyl sulfate solution used to denature test samples. Store at room temperature or 4°C.

4. Capture bead diluent: PBS solution containing protein. It is used to dilute capture beads prior to each experiment. Store at 4°C.

5. Detection reagent diluent: PBS solution containing protein. It is used to dilute the detection reagents prior to each experiment. Store at 4°C.

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