Buffers and Diluents

1. Wash buffer: 1X PBS solution, containing protein and detergent, used for wash steps and to resuspend beads for analysis. Store at 4°C.

2. Assay diluent: 1X buffered solution used to dilute the BD™ CBA Human Soluble Protein Flex Set Standards and to dilute test samples. Store at 4°C.

3. Capture bead diluent for serum/plasma samples: 1X PBS solution containing protein used to resuspend capture beads prior to testing serum or plasma samples. Store at 4°C.

4. Capture bead diluent for cell culture supernatant samples: 1X PBS solution containing protein used to dilute capture beads prior to testing cell culture samples. Store at 4°C.

5. Detection reagent diluent: 1X PBS solution containing protein used to dilute the detection reagents. Store at 4°C.

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