Calibrators and Standards

UniCAP-specific IgE calibrator strip (cat. no. 10-938601): human IgE in buffer, concentrations 0.35, 0.70, 3.50, 17.5, 50, and 100 kU/L. The IgE calibrators are traceable to the Second International Reference Preparation 75/505 of human serum IgE from WHO. Date and initial all calibrators upon opening. Each aliquot tube should be labeled with (1) substance name, (2) lot number, (3) date of preparation, (4) expiration date, and (5) any special storage instructions. Store at 2-8°C.

3. Procedure

3.1. Calibration Procedure

A six-point reference curve is set up along with the patient samples each time, and results are calculated by comparing the fluorescence of the unknown to that of the reference. The UniCAP 1000 Instrument measures specific IgE concentrations in kU/L and the instrument software automatically calculates the concentration.

3.2. Procedure

Follow the manufacturer's instructions.

4. Calculations

All calculations are preformed by the UniCAP operator software. For complete details, refer to the UniCAP 1000 system manual.

4.1. Other Commercial IgE Tests

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