Fc Fusion Protein Validation

1. 2-Mercaptoethanol (Sigma, cat. no. M7154).

2. Tris-HCl (Trizma; Sigma, cat. no. T5941): prepare 1 M solution, pH to 6.8.

3. Sodium dodecyl sulfate (SDS; EM Science, cat. no. 7910).

4. 2X Reducing buffer: each time a gel is run, add 50 pL of 2-mercaptoethanol (under fume hood) to 1 mL of buffer prepared as follows: 0.5 MTris-HCl (10 ml), glycerol (8 ml), 10% SDS (16 ml), 0.05% bromophenol blue (5 ml), and dH2O (37 ml).

5. 4-15% Tris-HCl gradient polyacrylamide gel (Bio-Rad, cat. no. 161-1104EDU).

6. Kaleidoscope prestained standard (Bio-Rad, cat. no. 161-0324).

7. Polyacrylamide Gel Electrophoresis (PAGE) apparatus (Bio-Rad Criterian Precast Gel System).

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