Histamine Release From Washed Human Leukocytes

5.1.2.1. Materials

1. 50-mL Polypropylene centrifuge tubes (Corning Inc. Corning, NY; cat. no. 25330).

2. 12 X 75-mm Polystyrene test tubes.

3. 2-mL Auto analyzer cups.

4. 1,4-piperazinebis (ethane sulfonic)-buffered saline containing 0.003% human serum albumin, 0.1% D-glucose (PAG) buffer.

5. 1,4-piperazinebis (ethane sulfonic)-buffered saline containing 0.003% human serum albumin, 0.1% D-glucose 10-M CaCl2 and 0.1 mM NaCl2 (PAGCM) buffer.

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