Instrument Setup Data Acquisition and Analysis

FACSComp software is used for the daily setup the FACSCalibur flow cytometer. CellQuest software is required for analyzing samples and formatting data for subsequent analysis using the FCAP Array software. Setup for the FACSArray bioanalyzer is required only once a month. Sample acquisition is automated on the FACSCalibur, using the carousel loader, whereas the FACSArray instrument acquires the samples directly from the microplate wells. The data are analyzed using the FCAP Array software. The outputs of the Flex Set assays are the standard curves for each assay (Fig. 5) and the tabulated results for each analytes.

3.3. Cell Signaling Bead Assay

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