Instruments and Equipment

1. 12 X 75 mm Glass or plastic culture tubes.

2. Aluminum foil.

3. P200 (20-200 pL) or equivalent with disposable tips.

4. P1000 pipet (200-1000 pL) or equivalent with disposable pipet tips.

5. Eppendorf adjustable digital pipet (2-10 pL and 10-1000 pL).

6. Microscope slides.

7. Cover slips.

8. Disposable transfer pipets.

9. 15-mL Conical centrifuge tubes with caps.

10. Disposable plastic Petri dish.

11. Sterile scissors/blades and forceps.

12. Parafilm.

13. 53-pMMesh, cut into 1-in. squares.

14. Vortex (MaxiMix or equivalent).

15. Biosafety hood.

16. Microscope.

17. Centrifuge with swinging bucket rotor.

18. Safety lids for centrifuge buckets.

19. 37°C Incubator.

20. 2-8°C Refrigerator.

21. Flow cytometer (BD FACS Canto).

22. Sample Prep Assistant II (SPAII; BD).

23. Wash-Lyse Assistant (BD).

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