3.4.1. Interpretative Guidelines

1. Examine CD45 fluorescence patterns on each specimen histogram. Problems with lysis should not affect results as the CD45-gating strategy focuses on leukocyte populations as positive and other "noise" as negative.

2. Note any abnormal scattering characteristics for the particular panel (i.e., dim CD45 populations).

Fig. 1. Algorithm for screening for hematological abnormalities by flow cytometry.
Fig. 2. Algorithm for the analysis of an abnormal population.
Fig. 3. The analysis and diagnosis of T-cell neoplasms.

3. Note any abnormal marking characteristics that may not be appropriate for the particular population selected or which may indicate additional testing to aid in diagnostic interpretation.

4. Always correlate flow cytometric results with morphology review. Any discrepancies should be investigated.

3.4.2. Interpreting Data

Record percent positive (include double marking) with respect to specific markers tested. Each completed specimen protocol sheet with history, viability, differential, fluorescent marking characteristics, and related histograms should be reviewed. Positivity and negativity for certain markers are characteristics for specific differentiation and growth of hematopoietic cells. Specific diagnosis can be rendered based on these characteristics. The following is an algorithm that can be used for the differential diagnosis of various hematopoietic neoplasma. However, final diagnosis should be based on integrating information from clinical history, histological and morphological evaluation, other laboratory data, and clinical history (1,6,8-10,12-19).

Fig. 4. The analysis and diagnosis of myeloid neoplasms.


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