Large Scale Production of Fc Fusion Proteins

1. Selective medium (see recipe under Subheading 2.4.).

2. T25 sterile tissue culture flasks, vented caps (Corning, cat. no. 430639).

3. T225 sterile tissue culture flasks, vented caps (Corning Costar, cat. no. 3001).

4. AHB complete spinner flask (Bellco, cat. no. 1967-03000).

5. 3 L Air stone sparger assembly (Bellco, cat. no. 1965-45003).

6. Aquarium air pump 4000 (Profile 4000 or similar).

7. HEPA-CAP 36 disposable filter capsule (Whatman, cat. no. 6702-3600).

8. 0.2-^m Bottle-top filter unit (Corning, cat. no. 430015).

9. 1 L Glass bottles, autoclaved.

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