Materials see Note

1. Goat anti-human Fab (horseradish peroxidase [HRP]-conjugated and alkaline phosphatase [AP]-conjugated) from AbD Serotec, Oxford, UK.

2. Mouse anti-His-tag antibody (HRP-conjugated) from Roche Applied Science, Penzberg, Germany.

3. Recombinant antibodies have all been selected from the HuCAL® GOLD antibody library at MorphoSys AG, Martinsried, Germany.

4. ECL Plus or ECL Advance (GE Healthcare, Little Chalfont, UK) and AttoPhos (Roche).

5. Buffer antigen retrieval (Dako, Glostrup, Denmark).

6. Antibody-diluent (Dako, Glostrup, Denmark, cat. no. S 2022).

7. 3,3'-diaminobenzidine Chromogen and DAB substrate (DakoCytomation, cat. no. K 3468).

8. Hematoxylin (Sigma, St. Louis, USA, cat. no. MHS16-500ML).

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