Procedure for Culturing and Drug Treatment of the K Cell Line

1. K562 cells are maintained in RPMI-1640 supplemented with 10% fetal bovine serum and antibiotics.

2. 1 X 106 K562 cells are plated in a six-well tissue culture plate and treated with dimethyl sulfoxide alone or 0.5 pM imatinib. Cells are incubated 18 h at 37°C in 5% CO2.

3. Cells are then washed two times with 1X PBS followed by lysing and permeabi-lization according to the protocol, starting with Subheading 3.3. An example of a result is shown in Fig. 3.

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