Purification of Fc Fusion Proteins

1. FPLC apparatus.

2. 5 mL HiTrap Protein A HP column (Amersham Biosciences, cat. no. 17-0403-01).

3. 1X Binding buffer: 0.15 Msodium chloride, 0.02 Mcitric acid in 1 L final vol, adjusted to pH 7.4 using HCl or NaOH.

4. 1X Elution buffer: 0.1 M sodium chloride, 0.02 Mcitric acid in 1 L final vol, adjusted to pH 3.0.

6. HiPrep 26/10 desalting column (Amersham Biosciences, cat. no. 17-5087-01).

8. UV spectrophotometer.

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