Purification of MAbs

1. Protein A- and Protein G-based commercially available purification kits are most commonly used to purify MAbs from ascitic fluids, supernatants, and other MAb preparations. These proteins are isolated from the cell wall of several microorganisms and naturally binds to the Fc portion of immunoglobulins from several mammalian species, including murine MAbs, at pH 8.2 (Protein A) and pH 5.0 (Protein G).

2. All murine IgG subclasses, except IgG1, bind strongly to both Protein A and Protein G and can be purified using either of these two systems. Because of better

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