The subject was selected because of its high state of preservation, making its organ systems discernible. Additionally, access to internal thoracic and abdominal cavities and the oral cavity was present. Data regarding the reflectance signatures for the following organs/tissues were collected and compared: lung, liver, heart, intercostal muscle, skin, and tooth. Composite reflective signatures were constructed from the raw data in order to examine the signatures for variation. The results indicated that each organ possessed a unique reflectance signature.
In this experiment, we also combined technologies to employ endoscopic guidance of the reflectance probe, to detect the impact of the endoscope guide light contamination on reflectance readings, and to verify probe position with radiography. As with all 2D radiographic exposures, two views were used to determine the location of a target within the 3D object. It is important to include these two views when reporting the reflectance raw data using anterior-posterior and lateral radiographs for probe location verification.
Using the liver reflectance data, we also demonstrated the impact of light from the endoscope guide and found that it caused considerable light contamination, leading to the determination that reflectance measurements should be made after endoscopically positioning the reflectance probe and then turning the light to the endoscope light guide off.
Reproducibility of the reflectance signature was also examined. We examined two separate lung reflectance signature graphs, with the second reading being taken after a rebooting and recalibration of the reflectance system. The data demonstrated a high correlation between the two measures.
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