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Pause E

ISTp

Pause E

Pause A

Figure 13.3. Location of ribosome pause sites along the amino acid sequence of membrane-bound chloroplast reaction center protein D1. The pause sites are numbered starting from the N terminus. Strong pause sites are designated by bold arrows, weaker pause sites with lines. Clusters of pause sites are denoted from A to F. a-Helical domains are boxed. The five vertical a-helices (I to V) correspond to membrane-spanning domains (see Chapter 18). The N-terminus of D1 is exposed to the stromal phase, whereas the C terminus is located in the lumen of the thylakoid. (Reproduced from J. Kim, P.G. Klein & J.E. Mullet, J. Biol. Chem. 266, 14931-14938, 1991, with permission).

Pause A

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