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Keto Resource Summary


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My Keto Resource Review

Highly Recommended

The writer presents a well detailed summery of the major headings. As a professional in this field, I must say that the points shared in this book are precise.

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The Building Block of Life Macromolecules

Several types of macromolecules are involved in forming a living system lipids, carbohydrates, proteins, and lastly, nucleic acids. Carbohydrates are polar molecules, possessing hydroxyl groups (OH bonds), and are thus soluble in water. The sugars, in particular (Fig. 9.2), are carbohydrates, which, dissolved in water, adopt a circular form. They are able to link with one another to form polymers, such as the polysaccharides. These can store energy and provide structure to organisms.

Starting in the Middle

Analyze theory-building how we will, we all must start in die middle. Our conceptual firsts are middle-sized, middle-distanced objects, and our introduction to diem and to everything comes midway in the cultural evolution of die race. In assimilating this cultural fare we are litde more aware of a distinction between report and invention, substance and style, cues and conceptualization, than we are of a distinction between die proteins and the carbohydrates of our material intake. Retrospectively we may distinguish the components of theory-building, as we distinguish the proteins and carbohydrates while subsisting on diem.

Endocytic Tracer Analysis

For the purpose of quantifying phagosome-lysosome fusion, the operational, albeit antiquated 3 definition of lysosomes as terminally endocytic compartments is often used. Tracer substances (fluorescent proteins or carbohydrates, ferritin, nanometer gold particles) are fed to phagocytes and are directed into the lysosomes by long chase times followed by feeding of the particle whose phagolysosome formation is to be investigated. A typical pulse-chase protocol would be 2-24 h of cultivation in presence of a fluorescent tracer, but absence of the phagocytic particle, followed by removal of uningested tracer, chase for 2-12 h and addition of the particle to be tested for 30 min. This is followed by a 1-2 h chase to allow the particle's phagosome to fuse with lysosomes and by sample fixation and preparation for (confocal) fluorescence microscopy. Alternatively, prelabeling of lysosomes with electron-dense markers such as 10 nm gold particles allows the exact determination in transmission...

There Are At Least Substantial Problems With The Standard Model

Third, in the standard model, both the symbiont and the host are heterotrophs. It is not evident why two heterotrophs would undergo a symbiotic relationship to begin with they would be competitors, not natural partners. Numerous and fascinating endosymbiotic relationships of prokaryotes living within eukaryotic cells are known, for example among the deep-sea tubeworms, which cannot eat for lack of a gut their symbiotic bacteria are chemoautotrophs (not heterotrophs) that make carbohydrates for their hosts from CO2 via H2S-dependent chemosynthesis (Dubilier et al. 2001).

The stumbling block of geometry

In terms of energy, a bacterial cell with double the 'normal' dimensions will produce half as much ATP per unit volume, while being obliged to divert more energy towards replicating the cellular constituents, such as proteins, lipids, and carbohydrates, that make up the extra cell volume. Smaller variants, with smaller genomes, will almost invariably be favoured by selection. It is therefore hardly surprising that only a handful of bacteria have achieved a size comparable with eukaryotes, and these exceptions merely prove the rule. For example, the giant sulfur bacterium Thiomargarita namibiensis (the 'sulfur pearl of Namibia'), discovered in the late 1990s, is eukaryotic in size 100 to 300 microns in diameter (0.1 to 0.3 mm). Although this caused some excitement, it is actually composed almost entirely of a large vacuole. This vacuole accumulates raw materials for respiration, which are continually washed up and swept away by the upwelling currents off the Namibian coast. Their giant...

The road less travelledfrom bacteria to eukaryotes

Eukaryotic cells and bacteria are constructed from essentially the same building materials (nucleic acids, proteins, lipids, and carbohydrates). They have exactly the same genetic codes, and very similar membrane lipids. Clearly they share a common inheritance. On the other hand, the eukaryotes are different from bacteria in virtually every aspect of their structure. Eukaryotic cells are, on average, 10 000 to 100 000 times the volume of bacteria, and contain a nucleus and many membranes and organelles. They generally carry orders of magnitude more genetic material and fragment their genes into short sections, in no particular order. Their chromosomes are straight rather than circular, and are wrapped in histone proteins. Most reproduce by sex, at least occasionally. They are supported internally by a dynamic cytoskeleton and may lack an external cell wall, which enables them to scavenge food and ingest whole bacteria.

GH and GHR as a Model System of Coevolution

Mammalian GH plays the role of a central endocrine regulator, controlling many different biochemical pathways (e.g., the metabolism of proteins, carbohydrates, and lipids). In humans, GH is also known to be involved in diabetes and to play a major role in carcinogenesis. Abnormal levels of GH directly induce specific phenotypes dwarfism when hyposecreted and gigan-tism when hypersecreted, before puberty. In adults, hypersecretion of GH caused by pituitary adenomas leads to a condition, known as acromegaly, distinguished by large fingers, hands, and feet (see Okada and Kopchick, 2001 for a recent review).

Problems specific to spacecraft

Exposing organisms to hard vacua leads to their desiccation through water evaporation or ice sublimation, and the irreversible polymerization of carbohydrates, lipids and nucleic proteins in spores and living bacteria. These Maillard reactions occur relatively slowly, with spores of Bacillus subtilis needing a D10 duration of the order of 10 days when exposed to low pressures in the range of 10 Pa (Horneck, 1993). Such durations are comparable to recent planetary missions and so exposure to vacuum does not in itself cause a significant reduction in a

The Sugar Code The Third Dimension

As implied by referring to a code system, information stored as sequence and shape will have to be grasped. Translating and transmitting it into intended responses is the task of decoding devices. They should specifically recognize coding units established by glycans. Thus, in addition to physicochemically serving roles to control folding, oligomerization and access of proteolytic enzymes, as already mentioned (Drickamer and Taylor, 1998 Gagneux and Varki, 1999 Reuter and Gabius, 1999 Sharon and Lis, 1997 Varki, 1996), oli-gosaccharides in glycan chains can be likened to the postal code in an address to convey distinct messages read by suitable receptors. These carbohydrate-binding proteins are classified into enzymes responsible to assemble, modify, and degrade sugar structures, immunoglobulins homing in on carbohydrates as antigens, and, last but not least, lectins. Evidently, the third class encompasses all carbohydrate-binding proteins, which are neither antibodies nor are they...

Inherency where is the ground plan in evolution

The navigation of protein hyperspace Life, then, is full of inherencies. We might legitimately enquire whether there is anything in the human condition that could prefigure some future level of complexity that with the virtue of hindsight will, no doubt, seem to have been inevitable, but to us remains unimaginable. Yet whatever privileges exist for untrammelled speculation, there is a story to be told which will occupy the rest of this book. My critics will, I imagine, complain at its eclectic, if not unorthodox, nature and given that the topics covered will include such matters as extraterrestrial amino acids and ants pursuing warfare it is advisable to try to explain the underlying thread of the argument. Here we can do no better than to look at a stimulating and thoughtful essay written by Temple Smith and Harold Morowitz,10 which is an exploration of the tension between the predictabilities of physical systems and the seemingly contingent muddle that we call history. In brief, and...

The Brain And The Chariot

There are many other examples in nature of this sort of evolutionary development. To take an almost random case, consider why plants are green. Green-plant photosynthesis utilizes light in the red and the violet parts of the solar spectrum to break down water, build up carbohydrates and do other planty things. But the sun gives off more light in the yellow and the green part of the spectrum than in the red or violet. Plants with chlorophyll as their only photosynthetic pigment are rejecting light where it is most plentiful. Many plants seem belatedly to have noticed this and have made appropriate adaptations. Other pigments, which reflect red light and absorb yellow and green light, such as carotenoids and phycobilins, have evolved. Well and good. But have those plants

Floral Relationships of Bees

Sugars in nectar are the principal source of carbohydrates in bees' diets. Nectar is eaten by adults as an energy source and mixed with pollen to make larval food. Nectar also contains some amino acids, and thus may also contribute toward a bee's nitrogen metabolism. Nectar for regurgitation into brood cells or for storage is carried to the nest in the crop.

The Asymmetry of Life

Forms, designated as d or l.4 Natural amino acids, the components of proteins (enzymes), are found in the L-form such as L-alanine, L-valine, L-leucine, L-aspartic acid, and others. Sugar molecules are - in contrast to this - biosynthesised in the right-form for implementation into nucleic acids. D-ribose and D-deoxyribose are used in ribonucleic acid RNA and deoxyribonucleic acid DNA respectively, and D-glucose in glycogen, starch, and cellulose. The mirror image structures of amino acids (called D-enantiomers) are not tolerated for the molecular architecture of proteins in a similar selective way, mirror image sugars (L-carbohydrates) do not contribute to the molecular construction of the nucleic acids RNA and DNA. In natural sciences, this widely distributed phenomenon is called biomolecular asymmetry.

Stegosaurus Second Brain

The much-ridiculed brain of Stegosaurus was indeed small analyses of braincase endocasts reveal that it possessed a volume of about 60 cm3. However, its olfactory bulbs were large compared to the rest of the brain, suggesting that Stegosaurus may have had an acute sense of smell. An oft-told fact about Stegosaurus is that its small brain was aided by a second brain, located in the hip region, which helped control the motor functions of the tail and legs. This story began in the late nineteenth century with O. C. Marsh's observation of a large space in the sacral vertebrae. Later speculation led to the hypothesis that this space held a nerve bundle that operated the posterior half of the animal, which was communicated as a second brain in the sacrum. Unfortunately, this popularized label was repeated for decades in both serious and trivial publications that mentioned Stegosaurus. Later, a more thorough analysis revealed that the sacral spaces had more than enough volume to allow the...

Biomineralization and Biochemistry of Bones and Other Hard Parts

But before biomineralization can occur, certain elements must be fixed through the interactions of biomolecules. Among these biomolecules are nucleic acids, such as deoxyribonucleic acid, DNA, and ribonucleic acid, RNA lipids, which are fatty molecules carbohydrates, also known as sugars and proteins. No undisputed evidence of preserved nucleic acids, lipids, or carbohydrates of dinosaurs has been documented. Indeed the conventional wisdom has been that very few or none of the original biomolecules of dinosaur bones have been preserved. Many biomolecules degrade quickly, even on a human time scale. This is why any report of their discovery in dinosaur bones is greeted with skepticism. However, recently reported results of detailed analyses, carried out on dinosaur bones during the past 20 years, have provided some evidence for the presence of proteins and other soft tissues. Because these contributed to the development of bones, connective tissues, and muscles in dinosaurs, the...

The Sugar Code Basic Principles

Currently, carbohydrates have their main place in textbooks in chapters on energy metabolism and cell wall composition. The regular repetitive arrangement of monosaccharides in plant, insect, fungal, or bacterial cell walls or coats seduces to underestimate the other inherent talents of carbohydrates. Amazingly, they are readily discernible when closely looking at a simple structural representation (Fig. 1). Each monosaccharide offers various hydroxyl groups for oligomer formation by glycosidic bonds including the anomeric Cl-position. In contrast to nucleic acids and proteins branching of chains is a common feature of the glycan part of cellular glycoconjugates (glycoproteins, glycolipids). Taking stock of the peculiarities of monosaccharide structure the total number of isomer permutation for a hexamer with an alphabet of 20 letters (monosaccharides) reaches the staggering number of 1.44 x 1015 (Laine, 1997). Under the same conditions only 6.4 x 107 (206) structures can be devised...

The Mitochondrial Theory of Ageing

Denham Harman, pioneer of free radicals in biology, first proposed the mitochondrial theory of ageing in 1972. Harman's central point was simple the mitochondria are the main source of oxygen free radicals in the body. Such free radicals are destructive, and attack the various components of the cell, including the DNA, proteins, lipid membranes, and carbohydrates. Much of this damage could be repaired or replaced in the usual way by the turnover of cell components, but hotspots of damage, most notably the mitochondria themselves, would be harder to protect simply by consuming dietary antioxidants. Thus, spake Harman, the rate of ageing and the onset of degenerative diseases should be determined by the rate of free-radical leakage from mitochondria, combined with the cell's innate ability to protect against, or repair, the damage.

Terraforming the Planets

How would we do it In the case of Venus, as we saw in Chapter 12, there is a crushing atmosphere, composed largely of carbon dioxide, and a searing surface temperature in excess of 900 degrees F. It would seem to be a formidable task indeed to convert this environment into one in which men could live and work without enormous technological assistance. But there is a bare possibility of reengineering Venus into a quite Earth-like place, a possibility I suggested with some caution in 1961. The method assumes that the high surface temperature is produced by a greenhouse effect involving carbon dioxide and water, a conjecture that is much more plausible now than it was then. The idea is simply to seed the clouds of Venus with a hardy variety of algae - a genus called Nostocacae was suggested - which would perform photosynthesis in the vicinity of the clouds. Carbon dioxide and water would be converted into organic compounds, largely carbohydrates, and oxygen. The algae would, however, be...

Sources of amphiphilic molecules

Two possible sources of organic compounds on a primitive planetary surface are delivery during late accretion, followed by chemical evolution, and synthesis by geochemical processes in the early atmosphere or hydrosphere. Early investigations focused on chemical synthesis of monomers common to the primary macromolecules involved in living systems, with the goal of determining whether it was possible that biologically relevant compounds were available on the primitive Earth. Most of these studies emphasized water-soluble compounds such as amino acids, nucleobases, and simple carbohydrates. The classic experiments of Miller (1953) and Miller and Urey (1959) showed that amino acids such as glycine

Summary and Outlook

DNA and RNA aptamers can, however, also recognize carbohydrates possessing exclusively hydroxyl functions and form with them stable complexes with Kds in the nanomolar range. Selection leads to more specific aptamers when additional substituents with a potential to participate in ionic interactions are integrated into carbohydrate and or nucleic acid structures. In addition, functional groups capable of undergoing hydrogen bond interactions common to carbohydrates are important.

The Role of the Cell

These molecules being scattered within the solvent. The cell membrane separates these molecules from the exterior medium. At the centre of a cell, DNA molecules store the genetic information they are surrounded by a solution of proteins and amino acids, and are protected by the cellular membrane, itself consisting of lipids and proteins, carbohydrates and amino acids. The cell is the basic structure of all living organisms that we know about, whether we are dealing with monocellular organisms or complex systems such as humans, who each contain some 1012 individual cells. Viruses do not consist of cells, but parasitize them to achieve their own life-cycle.


Dentition not only yields information about age at the time of death but can also give researchers clues regarding the dietary habits of an individual or populations of people. Wear patterns in younger individuals may suggest that sand inadvertently mixed in with the diet. High dental attrition at younger ages suggests a diet high in carbohydrates. Well-preserved teeth with little wear and little attrition may suggest a higher-status individual. Complete dental surveys can be conducted using the VE. In the case of dental attrition, the VE can provide images of the mandibular and maxillary surfaces to document to what degree reabsorption has occurred, indicating when the tooth or teeth were lost. Additionally, horizontal growth arrest lines can be seen on permanent teeth, suggesting past systemic infectious processes. Abscesses with bone loss can be seen, suggesting that poor dental


Nutritional analysis of Indri indri's top ten foods found them to be significantly lower in fats and water-soluble carbohydrates (sugars) than the top ten food items for the sympatric Propithecus diadema (Powzyk, 1997 Powzyk and Mo wry, 2003). P. diadema's top ten food items were an assortment of flowers, fruit seeds, whole fruits, and immature leaves (Powzyk, 1997), so the nutritional differences between the two indriids' diets were not surprising. Protein (crude, bound, available) levels were slightly higher for P. diadema's preferred foods, although not significantly (Powzyk and Mowry, 2003). Both Indri and P. diadema ate high-fiber diets, especially when compared to less folivorous primates. For example, Indri's preferred foods at Mantadia had a mean neutral detergent fiber (NDF) value of 56 dry weight compared to 31-34 for foods eaten by chimpanzees (Pan troglodytes schweinfurthi), blue monkeys (Cercopithecus mitis stuhlmanni), red-tailed monkeys (C. ascanius schmidti), and...

Metabolic State

Modifications in respiratory state from aerobic to microaerophilic to anaerobic as murine infection continues 33 . The changing metabolic and respiratory state of intracellular bacteria is best exemplified, however, in bacteria that cycle between vegetative and replicative stages, such as Chlamydia trachomatis and Legionella pneumophila. The chlamydial elementary body (EB), which survives extracellularly and is responsible for cell invasion, differentiates into the reticulate body (RB), which replicates intracellularly, before redifferentiation back into EB for release and reinfection. Shaw et al. 34 followed the gene expression profile of C. trachomatis after epithelial cell infection, and identified sets of genes regulated at early mid and late stages of infection. The early genes encode functions involved in the establishment of the chlamydial inclusion body and the acquisition of nutrients the mid-stage genes for the rapid multiplication of bacteria and late stage genes, the...

Agricultural losses

Not content with attacking human beings and adversely affecting the fauna of their adopted environment, invasive ants also wreak havoc among the flora. To meet their energy needs and build up calories, they require food rich in carbohydrates, which is why they go for flower nectar, pre-empting bees and thus preventing them from acting as pollinators. Argentine ants also enjoy feeding on buds, which devastates fig and orange groves in California. Also prevalent in the southern states of America, Solenopsis invicta fire ants destroy electrical equipment and irrigation systems. In addition, their nests are in the shape of mounds, which reduce the amount of land available for crops and, by making the terrain they colonize uneven, they damage agricultural machinery. In India, there are other tropical fire ants, Solenopsis geminata, whose depredations are mainly carried out on crops of cucumbers, tomatoes, potatoes, and cotton. Wherever they are to be found, these pests are enormously...

Cellular Delivery

Potency of such conjugates significantly by agents that are known to disrupt endosomes and or lysosomes, such as chloroquine or certain photosensitizers (Folini et al. 2003). Specific cellular targeting is also possible using e.g. cancer cell-specific peptides (Mier et al. 2003) or other ligands, such as carbohydrates, that bind to specific cell receptors (van Rossenberg et al. 2003 Hamzavi et al.

The ground floor

At a fundamental level matters seem to be rather simple. The principal elements required for life, at least as we know it, are carbon, hydrogen, oxygen, nitrogen, and phosphorus. These are all readily available, and carbon certainly has an almost uncanny knack of arranging itself in configurations that are both flexible and robust. Not that we should fail to consider alternatives. Iris Fry, for example, has an interesting discussion of both the definitions of life and the likelihood of radical alternatives that regard the 'carbaquist' (i.e. carbon plus water) foundation as hopelessly parochial.9 An ever-popular topic in this area is whether the element silicon, whose most familiar natural manifestation is probably as quartz crystals, could ever act as a substitute for carbon in providing the backbone of molecular architecture, be it in some sort of equivalent to the carbohydrates, lipids, or proteins of life. Silicon certainly possesses some advantages over carbon, such as its much...

Historical Matters

Jan Muller Palynologist

Figure 1.4 The vital, dynamic nature of pollen has long been recognized by humans in many cultures. The symbol shown above, from Silas John's Western Apache writing system (Basso and Anderson, 1973) bespeaks the ceremonial use of pollen by American Indians, symbolizing fertility, among other things. The pollen used by Silas John (a shaman) was apparently Typha, although the symbol would seem to represent a Zea tassel, the pollen of which was and is also used ceremonially by Indians. The jars illustrate the widespread modern use of pollen as a dietary supplement. Such pollen is sometimes harvested by human vacuum cleaners, sometimes taken from domesticated bees, which use pollen for hive nutrition. The human use is based on the vitamin, mineral, and nutritive (lipids, carbohydrates, amino acids) content of pollen. Apiaries sell untreated bee leg loads stripped from worker bees by a device at the hive entrance-hence the variously colored blobs in the jar, each being one leg-load....



Lichens are unique, double organisms that consist of two unrelated components, an alga and or cyanobacterium (pho-tobiont) and a fungus (mycobiont). The organisms that make up the lichen live in a close symbiotic relationship in which the photobiont gains mechanical protection, increased water availability, reduced desiccation, and an improved ability to obtain nutrients from the mycelium of the fungus. The fungus, in turn, gains organic nutrients synthesized by the photobiont(s) that is, a source of carbohydrates for growth.

Info About Pandas

Carbohydrates Excluding water, carbohydrates are the most abundant compounds in plants. As a storage form of photosynthetic energy, carbohydrates are quantitatively a significant source of food energy for herbivores (Van Soest, 1994). A diverse group, carbohydrates are classified according to chemical structure, which, in turn, influences how they are used by animals and how they are quantified analytically (NRC, 2003). Some carbohydrates (e.g. starch) are readily degraded by endogenous mammalian enzymes, such as salivary and pancreatic a-amylases. However, endogenous enzymes cannot degrade others, such as the structural carbohydrates cellulose and hemicellulose, where use depends on microbial fermentation by symbiotic gastrointestinal anaerobes yielding beneficial volatile fatty acids. Gastrointestinal adaptations which promote an environment conducive to this symbiotic relationship are pronounced in herbivores. However, as we have already indicated, these gastrointestinal...

Highfiber Herbivory

However, the relative delicacy of edaphosaur teeth suggests utilization of somewhat more frangible items their jaw and dental mechanics also appear sufficient for processing less indurated seeds and of megaspores, sporangia, and strobili, adding protein and readily digested carbohydrates to a high-cellulose diet.

Venus Is Hell

1977 Australian Nrl Squad

Forty miles above the surface is the thick cloud layer that we see from Earth. At least until recently, no one knew the composition of these clouds. I had proposed that they were constituted in part of water, a cosmically very abundant material, which could account for many but by no means all of the observed properties of the Venus clouds. But there were many other candidate materials proposed, among them, ammonium chloride, carbon suboxide, various silicates and oxides, solutions of hydrochloric acid, a hydrated ferric chloride, carbohydrates, and hydrocarbons. These last two materials were proposed by Immanuel Velikovsky in his speculative romance Worlds in Collision to provide manna for the Israelites during their forty years of wandering in the desert. The other candidate materials were proposed on somewhat firmer grounds. Yet each of them ran afoul of one or more of the observations.

Modern Animals

The three parts of an itemized water budget are gain, loss and storage (Shoemaker and Nagy, 1977). Positive storage occurs when an animal retains excess water, as do toads in their urinary bladder, and negative storage is synonymous with dehydration. The most common avenues of water gain are ingestion in the food or by drinking, osmosis through permeable skin, as in frogs and toads, and production of metabolic water de novo during oxidation of carbohydrates, fats and protein for energy production. Water leaves animals by evaporation across skin, lungs, or any permeable surface at a rate that is dependent on the temperature and humidity of the air, as well as the permeability of the evaporating surface. The factors that influence the rate of loss may differ, depending on whether evaporation is taking place from the lungs, skin, or eyes. Water is lost as part of the voided urine and feces, and in any glandular secretions released by the animal.

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