fly embryo mouse embryo

Figure 3.2

Analysis of c/s-regulatory elements and reporter genes

Animal genes often contain multiple independent elements that control gene expression in different places and/or different times during embryogenesis (for example, A, B, C). The c/s-regulatory elements are generally identified through their ability, when placed in c/s to a heterologous reporter gene and inserted back into a host genome, to control the pattern, timing, and/or level of gene expression. The most frequently employed reporter genes encode either (1) enzymes that can be detected in situ with chromogenic substrates or specific antibodies or (2) proteins that fluoresce in vivo.

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