Parentage Results

An example of a paternity assessment

We addressed parentage among 50 giant pandas in Chinese breeding centres using a panel of 17 previously described polymorphic microsatellite loci (Lu et al, 2001). A heterozygosity table was generated based on the genotypes obtained for 15 wild-born giant pandas in this study (Table 10.1). The number of alleles ranged from two to ten with an average of 5.4, and the heterozygosity ranged from 0.07 to 0.84 with an average of 0.63. In this study, Ame-^19 was inferred to reside on the X chromosome based upon the presence of two Ame-^19 microsatellite alleles in females and one allele in all males due to their carrying a hemizygous X chromosome. Ame-^70 has an unusually large size range with alleles ranging from 190 to 202 base pairs and 274 to 296 base pairs with a 72 base pair gap, probably due to an insertion/deletion event in the flanking sequence of the locus.

To illustrate the interpretation process for microsatellite genotypes, Table 10.2 presents a summary of paternity assessment for Tian

Table 10.1. Allelic diversity of 17 giant panda microsatellites

Microsatellite0

No. of alleles

Estimated heterozygosity

Allele range (base pairs)

Ame-|i5

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