The method described earlier is an example of how to detect selection from sequence data. To infer the functional significance of specific substitutions, however, requires detailed biochemical and structural data. The GH and GHR proteins provide an example with such desired data. They have been studied extensively for their biochemical properties, as well as evolutionary features. Using current technology of molecular biology and biochemistry, it may be even possible to experimentally determine whether a specific amino acid substitution has important functional consequences.
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