Inp

Outer membrane

NC D. Loop-grafted E. Dual display (eCPX)

Inner membrane

F. APEx

Periplasmic space ├ĄC Or ^N

FIGURE 2.2 Representative E. coli surface display platforms that have been used in protein engineering applications. An example of a binding interaction with a fluorescently labeled target is shown in B. Abbreviations: INP (ice nucleation protein), eCPX (enhanced circularly permuted OmpX), APEx (anchored periplasmic expression).

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