Established methods for library construction

The three major classes of library-construction methods applied to protein engineering are based on random mutagenesis (Figure 4.1A), recombination (Figure 4.1B), and site-directed diversification (Figure 4.1C). In their pure form, random mutagenesis and recombination mimic the random generation of variants during natural evolution, albeit at a much accelerated and tunable rate. In contrast, even in its most naive form, site-directed diversification requires judgment on which sites in a protein are most likely to yield beneficial mutations when diversified; thus, site-diversification methods are informed by a higher level of statistical and structure-based protein design.

0 0

Post a comment