Table B

Minimal Library size Required to Encode Top 48 Predicted sequences spanning Residues 324-334 in Galactose Oxidase

Library Type 324 326 329 330 333 334 Minimal Library size

Mix of defined- Oligonucleotides encoding variants #1-48 48

sequence oligos

Mix of defined- Oligonucleotides encoding variants #1-48 48

sequence oligos

Mix of codons

Asp

Arg

Arg

Arg

Arg

Glu

3x7x6x11x2x2 =

0 0

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