Identical and Independent binding sites

In this case all binding sites on the macromolecule are independent and have the same affinity for a particular ligand. The term "independent" means that the binding strength is independent of the fraction of the [So] binding sites that are occupied. "Identical" means that the equilibrium constant K for reaction (11.13) is the same for all sites. The law of mass action for reaction (11.13) is:

Conservation equations for ligands and sites are:

[Lo] is the total ligand concentration, bound plus unbound. The three equations, (11.14) — (11.16), contain three unknown concentrations. The solution is (see Problem 11.3a):

Getting Started With Solar

Getting Started With Solar

Do we really want the one thing that gives us its resources unconditionally to suffer even more than it is suffering now? Nature, is a part of our being from the earliest human days. We respect Nature and it gives us its bounty, but in the recent past greedy money hungry corporations have made us all so destructive, so wasteful.

Get My Free Ebook


Post a comment