O

o t3

Fig. 11.7 Effect of temperature on the folding - unfolding equilibrium of a protein in solution

Temperature is not the only parameter affecting protein-state transition. Other components in solution may have an equally profound effect. Figure 11.8 shows the effect of urea concentration in the solution containing the protein on the intensity of the fluorescence of the solution stimulated by the light of the appropriate wavelength. The straight-line portions at either end of the curve represent fluorescence of 100% folded state at low urea concentrations and 100% unfolded state at high urea concentrations. Increasing urea concentration has the same effect on protein stability as increasing temperature.

Getting Started With Solar

Getting Started With Solar

Do we really want the one thing that gives us its resources unconditionally to suffer even more than it is suffering now? Nature, is a part of our being from the earliest human days. We respect Nature and it gives us its bounty, but in the recent past greedy money hungry corporations have made us all so destructive, so wasteful.

Get My Free Ebook


Post a comment