The extent of ligand binding to a macromolecule is often measured spectroscopically. In the presence of a concentration [So] of the binding sites, the instrument detects the concentration of unbound ligand, [L], as a function of the (known) total ligand concentration, [Lo]. The latter is determined by adding ligand to a solution that does not contain the macromolecule so that the detection instrument responds to the total ligand concentration. The data are plotted as 1 -[L]/[Lo] versus [Lo] and compared to the theoretical model expressed by Eq (11.17). By combining Eqs (11.15) with (11.14), the quantity obtainable from experiment is expressed as:

which yields the fraction of ligand bounds to sites on the macromolecule:

Figure 11.9 is a plot of Eqs (11.20) and (11.17) for various values of K and [So] = 5 mM.

Getting Started With Solar

Getting Started With Solar

Do we really want the one thing that gives us its resources unconditionally to suffer even more than it is suffering now? Nature, is a part of our being from the earliest human days. We respect Nature and it gives us its bounty, but in the recent past greedy money hungry corporations have made us all so destructive, so wasteful.

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